On the presence of sulphate in pituitary lutropin

The presence of sulphate in the carbohydrate of pituitary lutropin from different species has been investigated using a biosynthetic approach. Pituitaries from rats, rabbits, goats, and buffaloes were incubated in the presence of 35SO4- and the 35SO4--labelled proteins in the tissue immunoprecipitated with a well characterized anti-sheep lutropin serum. The incorporation into immunoreactive lutropin was low in the case of rat, rabbit and goat pituitaries while, it was considerable in the case of buffalo pituitaries. Hence further characterization studies were carried out on 35SO4--labelled proteins of buffaloes. The physico-chemical, immunological and biological properties of radio-labelled buffalo pituitary material were shown to be similar to those of standard lutropin. Inin vitro conditions of incubations, most of the incorporation of 35SO4- was observed into tissue lutropin while under similar conditions of incubation, [14C]-amino acids were found to get incorporated mostly into medium lutropin. The physiologically specific releasing hormone, lutropin-releasing hormone was found to stimulate the release of 35SO4--labelled lutropin from the rabbit pituitaries into the medium. These results give indirect evidence that sulphate could be present in pituitary lutropin

Grey Relational Analysis and Anova to Determine the Optimum Process Parameters for Friction Stir Welding of Ti and Mg Alloys

The welding of Magnesium and Titanium and its alloys has continuously depicted a good challenge for designers and technologists. Ti and Mg alloys, particularly heat-treatable alloys, are difficult to join by fusion fastening techniques. The welding of dissimilar alloy such as Ti (Grade 2) and Mg (AZ91D) Alloy is an important problem during Friction Stir Welding (FSW). In this paper, the influence of Rotation speed (Rpm), Travel Speed (mm/min), Bottom Diameter Tool Pins (mm) and Tool Profiles of Ti and Mg alloy during FSW was investigated by Grey Relational Analysis and Anova was used to work out the foremost important Travel speed and feed rate affecting the Response. The primary and cooperation impact of the information factors on the normal reactions are examined. The expected values and measured values are genuinely close

Outcome of the Coating Thickness on the Tool Act and Process Parameters When Dry Turning Ti–6Al–4V Alloy: GRA Taguchi & ANOVA

In the primary days of Titanium Nitride tools, before coatings, tool manufacturers appreciated the tools would last elongate and scuffle cratering if they put a little bit of Titanium Nitride (TiN) in the combination when making the tool. This had the anticipated consequence, but the more TiN that was added, the feebler and more brittle the tool became. Then someone hit on the idea of applying a thin layer of TiN to the surface of the tool. This study results the Turning experiment conducted on the Ti–6Al–4V alloy of orthogonal array with Taughi grey relational analysis. Emphases on the optimization of turning process Constraints using the technique to get Min surface roughness (Ra), Roundness (s), Tool Wear and Cutting force in TIN with Different Coating Thickness by PVD Technique. A number of Turning experiments remained conducted mistreatment the L9 OA on All Gear Lathe. The experimentations remained achieved on Ti–6Al–4V alloy block of cutting tool of an CNMP120408-SM TN8025 of 12 mm diameter with cutting point 140 degrees, used throughout the experimental work beneath different Coating Thickness. Grey relational Analysis & ANOVA was used to work out the foremost important Cutting speed, feed rate, Depth of Cut and Different Coating Thickness of TIN with 50,100,150 μm by PVD Method which affecting the respons

Multi-Response Enhancement of Drilling Process Parameters for AM 60 Magnesium Alloy as per the Quality Characteristics utilizing Taguchi-Ranking Algorithm and ANOVA

: This investigation shows the improvement of Drilling parameters on AM-60 Mg alloy made with the help of Gravity Die Casting and with reactions upheld symmetrical cluster with Grey relational analysis - GRA. Which Focuses on the streamlining of Drilling constraints utilizing the system to get least surface Roughness (Ra), Tool Wear, Cutting Time, Power Requirement and Torque and Max MRR. Concentrates on the optimization of drilling constraints utilizing the procedure to get minimum surface roughness (Ra), Thrust Force, Burr size and Circularity Error. An amount of drilling experiments remained conducted mistreatment the L9 OA on CNC Machining Center. The trails remained achieved on Mg alloy block cutting tool of an ISO 460.1-1140-034A0-XM GC3 of 12 mm diameter with Tool Angle 140 degrees, used throughout the experimental work beneath dry cutting conditions. This experimental study results like Ra, TF, CE, and BZ were analyzed. GRA & ANOVA was utilized to effort out the principal essential Spindle speed, feed rate, Titanium Coated for Drill Bits (TiN, TiAN, TiCN) with 0.020 in Coating Thickness manipulating the Reaction. The essential and collaboration effect of the data influences on the ordinary responses remain analyzed. The standard qualities and projected values are truly near

Analysis of Product Quality Through Mechanical Properties and Determining Optimal Process Parameters of Untreated and Heat-Treated AISI 1050 Alloy during Turning Operation

AISI 1050 alloy has a wide range of applications and were subjected to machining operations. The purpose of the study is to find the optimum input plain turning process parameters at three levels, using L18 orthogonal array and grey relation analysis; and to investigate the mechanical and microscopic properties of the AISI 1050 alloy before (untreated, UT) and after annealing (AN) (heat-treatment, HT) process. The results of conducted experiments revealed the optimum process parameters as following for the untreated specimens: spindle speed at 3500 r.p.m., feed 0.08 mm/rev, depth of cut at 0.6 mm, insert corner radius at 4mm, and cutting fluid concentration at 12 %, are the most optimum conditions to obtain minimum power consumption for the untreated specimens. Whereas, a spindle speed at 3500 r.p.m., feed at 0.08 mm/rev, depth of cut at 0.6mm, insert corner radius at 4 mm, and cutting fluid concentration at 12 % are the optimum parameter values for the treated specimens

Performance Evaluation of Yield Function and Comparison of Yielding Characteristics of SS 304 in Annealed and Unannealed Conditions

Sheet metal forming forms in numerous industries like vehicle depend on the yielding of the sheet metals when strained. Yielding is portrayed by plastic flow of the materials when strained. The yield point if there should be an occurrence of uniaxial tension can be effectively decided from the pressure strain diagram, yet if there should arise an occurrence of multi axial Stresses it gets complicated. A connection between the principal stresses is required determining the conditions under which plastic flow occurs. This intricacy is tended to by the anisotropic yield capacities. Likewise, the tests used to acquire yield loci might be costly and time taking in such case these yield capacities end up being exceptionally viable. The yield criteria additionally help in deciding planar distribution of yield stresses and anisotropic coefficients, which gives a decent gauge of these mechanical parameters without having to through the pain of trial assurance. This project aims at using Hill 1948 criterion to obtain the Yield surface Diagrams for SS304 in annealed and original state and subsequently obtain the planar distribution of the uniaxial yield stress and anisotropic coefficient. Also, the performance evaluation of both the distributions will be done using accuracy index